8600 Rockville Pike TEV protease (EC 3.4.22.44, Tobacco Etch Virus nuclear-inclusion-a endopeptidase) is a highly sequence-specific cysteine protease from Tobacco Etch Virus (TEV). For example, we may use these cookies to determine if you have interacted with a certain page. Learn more and request a sample! ProTEV Plus cleavage efficiency remained at 94% in the presence of Tween 20, Triton X-100, IGEPAL, glycerol, urea, Protease Inhibitor Cocktail and guanidine for all concentrations tested (Figure 2). Thermo Scientific Leupeptin Protease Inhibitor reversably inhibits serine and cysteine proteases. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site. It can be used to cleave protein fusions that have been engineered to contain this seven-amino-acid sequence at the desired cleavage site. TEV Protease activity inhibition of by TEV Protease Inhibitor (IC50 46 nM). international site. Federal government websites often end in .gov or .mil. ProTEV Plus, an engineered form of TEV protease, is a highly specific proteolytic enzyme that cleaves fusion proteins at a specific TEV linkage site. E-64 is also known as N- [N- (L-3-trans-carboxyoxiran-2-carbonyl)-L-leucyl]-agmatine. It is a member of the PA clan of chymotrypsin-like proteases. official website and that any information you provide is encrypted In this study we test the enzymatic activity of ProTEV Plus in the presence of various compounds commonly found in protein purification protocols. Learn about our tools that are helping researchers develop diagnostics and vaccines for the SARS-CoV-2 virus. Trademarks. Enhanced enzyme stability for prolonged protease activity. Epub 2010 Sep 9. If solvent effect on enzymatic activity is a concern, Purchase these through your usual distributor. This TEV Protease Inhibitor Screening Kit (Fluorometric) (ab284516) (K843) offers a rapid, simple, sensitive, and reliable test suitable for high-throughput screening of MAO-A inhibitors. For simple, visual assay results, the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit includes a color-changing pH indicator for detection of SARS-CoV-2 nucleic acid amplification. Please refer to protocols. Reactivos e instrumentos para inmunologa, biologa celular y biologa molecular. 2022 Promega Corporation. NOT FOR USE IN DIAGNOSTIC PROCEDURES" For licensing inquiries, please contact partnerships@abcam.com, TEV Protease Inhibitor Screening Kit (Fluorometric). A verified email address is required to access the full functionality of your Promega.com account. This site needs JavaScript to work properly. 2022;2507:143-173. doi: 10.1007/978-1-0716-2368-8_8. Methods Mol Biol. Purification of Membrane Proteins Overexpressed in Saccharomyces cerevisiae. The gels were run for 1 hour at 200V and stained with SimplyBlue SafeStain (LifeTech, Cat.# LC6060). A master mix was prepared with all components except the ProTEV Plus and test compound. K843-100 | TEV Protease Inhibitor Screening Kit (Fluorometric). Protease reactions were incubated at 30C for 30 minutes. 2017;1586:221-230. doi: 10.1007/978-1-4939-6887-9_14. FOIA ProTEV Plus also contains a metal affinity tag, HQ tag, located at the N-terminus of the protein. V6101) is an engineered form of Tobacco Etch Virus (TEV) protease, a highly specific proteolytic enzyme that cleaves within a seven-amino-acid sequence. Like the serine proteases, TEV protease utilizes a "catalytic triad" of residues to catalyze peptide hydrolysis. Lundbck AK, van den Berg S, Hebert H, Berglund H, Eshaghi S. Anal Biochem. Inhibition occurs in the presence of 5 mM Zn 2+, 1 mM Cu 2+ and 10 mM Co 2+. For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome. Proteolytic cleavage of the tagged domain is carried out by incubation of the protein with rTEV protease. In the presence of a TEV Proteasespecific inhibitor, the cleavage . Panel B. Protease Inhibitors, Urea, Guanidine, IGEPAL, Triton X, Glycerol, Tween 20, SDS and DTT. However, in TEV protease the serine nucleophile of the conventional Ser-Asp-His triad is a cysteine instead. Please try again or contact Customer Service. This reduction in protease activity is suggestive of detergent-induced inhibition of rTEV. Zinc ions inhibit the activity of . We have observed that the efficiency of rTEV digestion decreases significantly in the presence of a variety of detergents utilized in purification, crystallization, and other biochemical studies of integral membrane proteins. Activity over a broad temperature (4C to 30C) and pH (6.0 to 8.5 . This inhibitor was the most potent TEV protease inhibitor synthesized, with nanomolar activity against the protease (IC 50 = 730 nM with 1 h of preincubation; Fig. Proteins are puried using affinity chromatography followed by buffer-exchange chromatography, to promote protein solubility and efficient tag re-moval. Legal and Trademarks 2011 Aug;78(2):139-42. doi: 10.1016/j.pep.2011.04.011. The protein is further puried using affinity Hello, Sign in. You have not verified your email address. The affinity tag is cleaved off by a specic tagged protease. Miladi B, Bouallagui H, Dridi C, El Marjou A, Boeuf G, Di Martino P, Dufour F, Elm'Selmi A. HHS Vulnerability Disclosure, Help Enrquez-Flores S, De la Mora-De la Mora JI, Flores-Lpez LA, Cabrera N, Fernndez-Lainez C, Hernndez-Alcntara G, Guerrero-Beltrn CE, Lpez-Velzquez G, Garca-Torres I. Appl Microbiol Biotechnol. All rights reserved. Please contact Customer Service to unlock your account. TEV Protease Assay Buffer 25 mL -20C TEV Protease 100 L points (T-20C TEV Protease Substrate 100 L -20C TEV Protease Inhibitor (2 mM) 20 L -20C Materials Required, Not Supplied These materials are not included in the kit, but will be required to successfully utilize this assay: 2 White 96-well plate with flat bottom. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the . Protease reactions were assembled as shown in Table 1. TEV Protease can recognize the cleavage site of Glu-Xaa- Xaa-Y- Xaa-Gln-(Gly/Ser) and cleaves between Gln and Gly/Ser. However, in TEV protease the serine nucleophile of the conventional Ser-Asp-His triad is a cysteine instead. TEV Protease activity is unaffected by the following protease inhibitors: aprotinin, benzamidine, leupeptin, pepstatin, and PMSF. Like the other cookies we use, strictly necessary cookies may be either first-party cookies or third - party cookies. Spectroscopy, Elemental & Isotope Analysis, Preclinical to Companion Diagnostic Development, Microbiological Media and Media Additives, Gel Electrophoresis Equipment and Supplies, Protease Inhibitors and Phosphatase Inhibitors, Pierce Protease and Phosphatase Inhibitor Mini Tablets, EDTA Free, Pierce Protease Inhibitor Mini Tablets, EDTA Free. Please try again or contact Customer Service. Activity and Specificity. Our customer and technical support experts are here to help! All tested concentrations resulted in 0% cleavage. PMC Note: Solvents used to solubilize the inhibitors might affect the enzymatic activity. Removal of a hexahistidine amino-terminal affinity tag has been characterized in the presence of 16 different detergents at concentrations above their respective CMCs. Search Affinity tags such as polyhistidine greatly facilitate recombinant protein production. In this study we test the enzymatic activity of ProTEV Plus in the presence of various compounds commonly found in protein purification protocols. Front Mol Biosci. Promega eNotes. Exploring the activity of tobacco etch virus protease in detergent solutions. Please check your network settings and try again. TEV protease can be used at high concentrations without the occurrence of non-specific proteolysis. For maximum convenience and value, columns and buffers are also available separately. All Rights Reserved. Epub 2022 Jan 29. Thermo Scientific Leupeptin Protease Inhibitor reversably inhibits serine and cysteine proteases. Agonists, activators, antagonists and inhibitors, Platform: Microplate (12 x 8 well strips). You can set your browser to block or alert you about these cookies, but some parts of our services will not work without them. Sun C, Liang J, Shi R, Gao X, Zhang R, Hong F, Yuan Q, Wang S. Protein Expr Purif. Activity over a broad temperature (4 . If incorrect, please enter your country/region into the box below, to view site information related to your country/region. Complete inhibition controls (+) contained 5% SDS, and no-inhibition controls () contained water. Figure 2. ab284516 has not yet been referenced specifically in any publications. Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs. E-64 is a cysteine protease inhibitor that was isolated from the mold Aspergillus japonicus TPR-64. Bands were quantitated using Image Lab Software. Your profile has been mapped to an Institution, please sign back for your profile updates to be completed. We use these cookies to remember your settings and preferences. It is a member of the PA clan of chymotrypsin-like proteases. Plate Readers, Fluorometers & Luminometers, Small Molecule Profiling and Assay Development, ProTEV Protease for Fusion Protein Processing. Clipboard, Search History, and several other advanced features are temporarily unavailable. Please try again or contact Customer Service. There was an issue with the password reset process. Inhibition of TEV Protease activity by TEV Protease Inhibitor (IC50 46 nM). Proteolytic cleavage of the tagged domain is carried out by incubation of the protein with rTEV protease. Identifcate This product is manufactured by BioVision, an Abcam company and was previously called K843 TEV Protease Inhibitor Screening Kit (Fluorometric). Incubate at room temperature for 15 min. We determined the percentage of HaloTag Standard Protein, which was comprised of the 34kDa HaloTag protein and 26kDa GST protein, cleaved by quantitating and comparing the bands of uncleaved HaloTag Standard Protein in each experimental reaction to those present in the water control reactions (Figure 1). A verification email has been sent to the primary email address associated with your account. The SDS gels were visualized using a Molecular Imager Gel Doc XR+ System and quantitated with Image Lab Software. Please try again or contact Customer Service. Publication Date: November 2013; tpub_136. Would you like email updates of new search results? Removal of the affinity tag necessitates inclusion of an engineered protease cleavage site. 2012 Mar;82(1):226-31. doi: 10.1016/j.pep.2012.01.005. British Pound Euro; Polish Zloty USD 7560 Apple Lane Baileys Harbor, WI 54202 (718) 513-2983; Sign in Register. The variable detergent sensitivity of proteases that are utilized for recombinant protein affinity tag removal. Figure 1. Click here for terms and conditions. GFP-Trap . Bethesda, MD 20894, Web Policies Table 3 lists the compounds tested and the concentrations at which ProTEV Plus shows 94% cleavage. Get 4 for 3 with code 4FOR3-E5TN2. Careers. Select Currency: USD. An official website of the United States government. We use these cookies to collect information about how you interact with our services and to help us measure and improve them. E-64 effectively inhibits various cysteine proteases, in particular: cathepsin K. cathepsin L. cathepsin S. E-64 also acts against other enzymes, such as: calpain. ProTEV Plus, an engineered form of TEV protease, is a highly specific proteolytic enzyme that cleaves fusion proteins at a specific TEV linkage site. 3d) and a k inact /K i value of . No non-specific cleavage has been observed under the . A commonly utilized protease for tag removal is tobacco etch virus (TEV) protease. It looks like you are having trouble logging in, please try our dedicated login page. Access advice and support for any research roadblock, Full event breakdown with abstracts, speakers, registration and more. 2022;2507:337-358. doi: 10.1007/978-1-0716-2368-8_18. This probably explains why TEV protease is resistant to many commonly used protease inhibitors. ProTEV Plus (Cat.# V6101) is an engineered form of Tobacco Etch Virus (TEV) protease, a highly specific proteolytic enzyme that cleaves within a seven-amino-acid sequence. Raran-Kurussi S, Cherry S, Zhang D, Waugh DS. For Research Use Only. Your browser does not have JavaScript enabled and some parts of this website will not work without it. There was an issue creating your account. To save your cart and view previous orders, sign in to your NEB account. Assay was performed following the kit protocol. We provide medical information and facilitate research collaborations. You have been idle for more than 20 minutes, for your security you have been logged out. A password reset email has been sent to the primary email address associated with your account. The individual reagents include AEBSF, aprotinin, bestatin . TEV protease is a highly sequence-specific cysteine protease. Methods Mol Biol. You have successfully reset your password. For some of the compounds analyzed, the highest acceptable concentration may be higher than indicated. Due to its high sequence specificity it is frequently used for the controlled cleavage of fusion proteins in vitro and in vivo. Promega Corporation Compatible with 10mM MgSO 4, MnCl 2 and CaCl 2 and up to 100mM EDTA. HaloTag Standard Protein (Cat.# G4491), a ~61kDa fusion protein containing HaloTag (34kDa) and glutathione-S-transferase (GST; 26kDa) separated by an optimized TEV cleavage site, was cleaved with ProTEV Plus protease. There was an issue verifying your email address. Methods Mol Biol. tev-protease-inhibitor-screening-kit-fluorometric-ab284516.pdf. Jaramillo-Martinez V, Ganapathy V, Urbatsch IL. 2008 Nov 1;382(1):69-71. doi: 10.1016/j.ab.2008.07.018. Thermo Fisher Scientific, Don't have an account ? Peptide Tags and Domains for Expression and Detection of Mammalian Membrane Proteins at the Cell Surface. K843-100 is the same size as the 100 test size of ab284516. Accessibility Our data indicate that half of the detergents tested reduce the activity of rTEV and that these detergents should be avoided or otherwise accounted for during rTEV digestion of recombinant integral membrane proteins. For others, we wont set them unless you accept them. Compatible with the following protease inhibitors: aprotinin, benzamidine, leupeptin, pepstatin, PMSF. If you don't see your country above, please visit our TEV Protease Inhibitor Screening Kit utilizes the ability of TEV Protease to cleave a synthetic Fluorescein-based peptide substrate to release fluorescein which can be easily quantified using a fluorometer or fluorescence microplate reader (Ex/Em = 490/560 nm). To test this hypothesis, we examined the effects of detergents upon the rTEV proteolytic digestion of a soluble fusion protein, alpha(1) platelet activating factor acetylhydrolase (PAFAHalpha(1)). . The concentration limits provided are general guidelines because we did not test the compound concentrations in small increments. Thermo Scientific Protease Inhibitors are small packages of individual protease inhibitor peptides and compounds for customized formulation or modification of protease inhibitor cocktails. An improved version of Tobacco Etch Virus (TEV) AcTEV Protease is highly site-specific, highly active and significantly more stable than native TEV protease, resulting in enhanced long-term activity. Account & Lists Returns & Orders. To protect your privacy, your account will be locked after 6 failed attempts. Your password reset link has expired. Lee E, de Paula MN, Baek S, Ta HKK, Nguyen MT, Jeong TH, Kim CJ, Jang YJ, Choe H. Int J Mol Sci. sharing sensitive information, make sure youre on a federal Novel Bacterial Production of Two Different Bioactive Forms of Human Stem-Cell Factor. We and our advertising partners use these cookies to deliver advertisements, to make them more relevant and meaningful to you, and to track the efficiency of our advertising campaigns, both on our services and on other websites and social media. Cart Note: You will not be able to access your account until your email is verified. Percent cleavage of HaloTag Standard Protein using ProTEV Plus in the presence of various compounds. There was an issue logging into your account. 1998-2022 Abcam plc. Here we show that ProTEV Plus can be used to cleave fusion proteins in the presence of various compounds common to protein purification protocols. Check your inbox to complete email verification. A commonly utilized protease for tag removal is tobacco etch virus (TEV) protease. MeSH Please note: All products are "FOR RESEARCH USE ONLY. Description. Thank you for verifying your email address. Is TEV Protease compatible with protease inhibitors? Two control reactions were used: 5% Sodium Dodecyl Sulfate (SDS), a known inhibitor of protease, was used to block cleavage, and water was used as a no inhibitor control. Not for use in diagnostic procedures. The assay is based on the fluorometric detection of H 2 O 2, one of the byproducts generated during the oxidative deamination of MAO substrate (Tyramine). Anderson, J. et al. (2008) ProTEV Protease for Fusion Protein Processing. Save 20% On GloMax Instruments! The solubility of integral membrane proteins is maintained by the formation of protein-detergent complexes (PDCs), with detergent present at concentration above its critical micelle concentration (CMC). Redeem this savings with your local representative through December 31st. TEV protease does not belong to serine protease family and is therefore not sensitive to common protease inhibitors, such as PMSF and AEBSF (1mM), TLCK (1mM), Bestatin (1mg/ml), pepstatin A (1mM), EDTA (1mM), and E-64 (3mg/ml), and complete" protease inhibitor cocktail (Roche). An improved version of Tobacco Etch Virus (TEV) AcTEV Protease is highly site-specific, highly active and significantly more stable than native TEV protease, resulting in enhanced long-term activity. BP TM TEV Protease has a specific activity of at least 10,000 units/mg, using the conventionally defined activity unit (One unit cleaves >85% of 3 g control substrate in 1 h at 30 o C). TEV is available in a recombinant form (rTEV) and frequently contains its own polyhistidine affinity tag for removal after use in enzymatic digestion. government site. Get in touch with a nearby distributor or sales representative. . Store at -20C. We have determined that a variety of compounds commonly used with protein purification protocols do not inhibit ProTEV Plus cleavage reactions. Take a look at our BETA site and see what weve done so far. There was an issue sending the verification email. Please try again or contact Customer Service. TEV Protease Inhibitor Screening Kit (Fluorometric) Toggle menu. Get resources and offers direct to your inbox. Protein Expr Purif. BioVision's Inhibitor Screening Kit utilizes the ability of TEV Protease to cleave a synthetic Fluorescein-based peptide substrate to release fluorescein which can be easily quantified using a fluorometer or fluorescence microplate reader (Ex/Em = 490/560 nm). In the presence of a TEV Proteasespecific inhibitor, the cleavage of the substrate is reduced/abolished resulting in decrease or total . The HQ tag consists of 6 amino acids (HQHQHQ), and allows the protease to be immobilized on nickle affinity resins and removed from the cleavage reaction. There was an error processing your request. Create Account. Enter the email address you signed up with and we'll email you a reset link. 2021 Jun 14;22(12):6361. doi: 10.3390/ijms22126361. Please enable it to take advantage of the complete set of features! Tobacco etch virus protease retains its activity in various buffers and in the presence of diverse additives. After that, you will need to contact Customer Service to unlock your account. Our latest RUO kit, the Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit, enables high throughput workflows for real-time detection of SARS-CoV-2 nucleic acid using hydrolysis probes. The master mix was dispensed to reaction tubes, and the appropriate compounds (Table 2) were added before finally dispensing ProTEV Plus. Are you doing COVID-19 related research? ProTEV Plus protease performs over a broad pH and temperature range, and has been shown to be highly specific in cleaving fusion proteins with a TEV recognition site both in solution as well as when the fusion protein is bound to affinity resins (1) . Cholesterol Is a Dose-Dependent Positive Allosteric Modulator of CCR3 Ligand Affinity and G Protein Coupling. You've created a Promega.com account. (TEV) protease cleavage site that resides between the coding . Some of these cookies are essential for our website to work. Following digestion, 30l of each reaction was added to 10l of 4X SDS gel-loading buffer, heated to 95C for 5 minutes, and 20l was loaded onto a 420% precast SDS gel (Bio-Rad, Cat.# 345-0033). Please let us know so that we can cite the reference in this datasheet. We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. ProTEV Plus digestions containing NaCl, imidazole, glutathione or MgCl2 resulted in a variable amount of cleavage inhibition. The .gov means its official. To protect your privacy, your account has been locked after 6 failed login attempts. Please try again or contact Customer Service. ThisTEV Protease Inhibitor Screening Kit (Fluorometric) (ab284516) (K843) offers a rapid, simple, sensitive, and reliable test suitable for high-throughput screening of MAO-A inhibitors. HT-GST protein (10g) was digested with 5 units of ProTEV Plus in 50l reactions for 30 minutes at 30C. Protein Expr Purif. In the presence of a TEV Proteasespecific inhibitor, the cleavage of the substrate . This probably explains why TEV protease is resistant to many commonly used protease inhibitors. TEV protease is inhibited by reaction buffers containing >40% Glycerol. Next Generation Sequencing Library Preparation, DNA Assembly, Cloning and Mutagenesis Kits, Supporting Infectious Disease Research & Development. If the fusion protein sample contains > 2 M urea, > 0.5 M Guanidine hydrochloride, > 50 mM imidazole, pH values below 6 or above 9, or cysteine protease inhibitors then it will be necessary to dialyze the fusion protein before TEV Protease cleavage. Removal of Affinity Tags with TEV Protease. For example, we may use these cookies to remember your language preferences. HTP adaptable assay kit to screen, study and characterize potential inhibitors of TEV Protease.Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. Epub 2011 Apr 24. For Inhibitor Control (IC), add 1 l TEV Protease Inhibitor and 9 l TEV Protease Assay Buffer into TEV Protease enzyme well(s). By creating an account, you confirm that you accept the. The N-terminal residue (A, S or C) can be exposed by the TEV protease during protein purification. The site is secure. Please contact us to place your order, or try again later. Assay was performed following the kit protocol. Disclaimer, National Library of Medicine The assay is based on the fluorometric detection of H2O2, one of the byproducts generated during the oxidative deamination of MAO substrate (Tyramine). Product Overview :TEV Protease Inhibitor Screening Kit utilizes the ability of TEV Protease to cleave a synthetic Fluorescein-based peptide substrate to release fluorescein which can be easily quantified using a fluorometer or fluorescence microplate reader (Ex/Em = 490/560 nm). Unable to load your collection due to an error, Unable to load your delegates due to an error. Bookshelf In practice, 10,000 units (1mg) of BP TM TEV Protease cleaves >90% of 100 mg of a control target protein at 4 o C in 16 hours. Congratulations! There are currently no Customer reviews or Questions for ab284516.Please use the links above to contact us or submit feedback about this product. Enter your username and we'll send a link to reset your password. Guaranteed product quality, expert customer support. The presence of NaCl (>10mM), imidazole (>5mM), glutathione (>1mM) and MgCl2 (>1mM) decreased ProTEV Plus cleavage efficiency to less than 50%. Publishing research using ab284516? TEV Protease activity is unaffected by the following protease inhibitors: aprotinin, benzamidine, leupeptin, pepstatin, and PMSF. ProTEV Plus functions over a broad pH and temperature range. Before This kit utilizes the ability of TEV Protease to cleave a synthetic Fluorescein-based peptide substrate to release fluorescein to screen/study . Panel A. Imidazole, NaCl, Glutathione and MgCl2. There was an issue resetting your password. It can be used to cleave protein fusions that have been engineered to contain this seven-amino-acid sequence at the desired cleavage site. Terms and Conditions Epub 2012 Jan 20. Like the serine proteases, TEV protease utilizes a "catalytic triad" of residues to catalyze peptide hydrolysis. ProTEV Plus also contains a metal affinity tag, HQ tag . eCollection 2021. and transmitted securely. Contact your local subsidiary or distributor. Epub 2008 Jul 26. Enhanced enzyme stability for prolonged protease activity. Our records indicate that this email address is already registered. The TEV Protease Inhibitor Screening Kit (Fluorometric) LS-K719 is a simple, fast fluorometric assay for the HTS screening and characterization of TEV Protease inhibitors. Highly specific cleavage activity. TEV Protease inhibitors. TEV Protease activity is unaffected by the following protease inhibitors: aprotinin, benzamidine, leupeptin, pepstatin, and PMSF. 2011 Jan;75(1):75-82. doi: 10.1016/j.pep.2010.08.012. Kits are available for total RNA purification, plasmid miniprep, gel extraction, and DNA & RNA cleanup. ProTEV Plus functions over a broad pH and temperature range. A new tagged-TEV protease: construction, optimisation of production, purification and test activity. proteolysis with several protease inhibitors. Reactions were separated using 420% SDS-PAGE at 200V for 1 hour. *Indicates the maximum concentration tested for a given compound. Wish Lists; Compare ; Recently Viewed . Welcome to TELOS The Electronic Library of Life Science! 10% glycerol, add freshly 1 mM DTT, 1 mM PMSF, and 1x protease inhibitors). Description. TEV Protease Inhibitor Screening Kit utilizes the ability of TEV Protease to cleave a synthetic Fluorescein-based peptide substrate to release fluorescein which can be easily quantified using a fluorometer or fluorescence microplate reader (Ex/Em = 490/560 nm). 2022 Feb;106(4):1475-1492. doi: 10.1007/s00253-022-11786-5. We use these cookies to ensure our site functions securely and properly; they are necessary for our services to function and cannot be switched off in our systems. Haslem L, Brown M, Zhang XA, Hays JM, Hays FA. Monarch Nucleic Acid Purification Kits are optimized for maximum performance and minimal environmental impact. 2021 Aug 20;8:724603. doi: 10.3389/fmolb.2021.724603. Please sign back in to continue your session. Stay notified of Promega events, products and news. Thermo Scientific Protease Inhibitors are small packages of individual protease inhibitor peptides and compounds for customized formulation or modification of protease inhibitor cocktails. Improved yield, stability, and cleavage reaction of a novel tobacco etch virus protease mutant. PF-00835231, a 3CL protease inhibitor, has exhibited potent in vitro antiviral activity against SARS-CoV-2 as a single agent. TEV is available in a recombinant form (rTEV) and frequently contains its own polyhistidine affinity tag for removal after use in enzymatic digestion. Please request another reset link. The https:// ensures that you are connecting to the To find out more about cookies and how to manage cookies, read our Cookie Policy. They are usually only set in response to actions made by you which amount to a request for services, such as logging in, using a shopping cart or filling in forms. This article highlights use of ProTEV Protease, an engineered protease with greater stability and prolonged activity over native TEV protease. Percent cleavage was calculated by Image Lab Software by comparing the bands of the partially digested HaloTag Standard Protein to the band of undigested HaloTag Standard Protein. 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